منابع مشابه
Mycobacterium tuberculosis HspX/EsxS Fusion Protein: Gene Cloning, Protein Expression, and Purification in Escherichia coli
Background: The purpose of this study was to clone, express, and purify a novel multidomain fusion protein of Micobacterium tuberculosis (Mtb) in a prokaryotic system. Methods: An hspX/esxS gene construct was synthesized and ligated into a pGH plasmid, E. coli TOP10 cells were transformed, and the vector was purified. The vector containing the construct and pET-21b (+) plasmid were digested ...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 2004
ISSN: 0022-1554,1551-5044
DOI: 10.1369/jhc.3a6221.2004